Journal: Kidney360
Article Title: Renal Single-Nuclear Transcriptomics Identifies Novel Therapeutic Targets in a Preclinical Model of CKD
doi: 10.34067/KID.0000000945
Figure Lengend Snippet: Renal protein expression of randomly selected DEGs in endothelial, proximal tubular, and connective tubular cells. (A) Violin plots (A) and UMAPs (B) showing the expression of KDR , vWF , LAMA3 , ICAM1 , and PTGIS in endothelial cells of normal and CKD pigs ( n =3/group). (C) Representative immunoreactivity and quantification for vWF , LAMA3, and ICAM1 in endothelial cells. (B) UMAPs (A) and violin plots (B) showing the expression of CTSC and TSPAN in proximal tubular (top) and GHSR , SLIT2 , and DMD in connecting tubular (bottom) cells of normal and CKD pigs ( n =3/group). (C) Representative immunoreactivity for TSPAN18 in proximal tubular cells and GHSR in connective tubular cells. * P < 0.05 versus normal. EC, endothelial cells; GHSR, growth hormone secretagogue receptor; ICAM1, intercellular adhesion molecule 1; LAMA3, laminin subunit alpha 3; TSPAN , Tetraspanin; TSPAN18, Tetraspanin 18.
Article Snippet: Overlapping genes in each cell type were displayed in heatmaps, violin plots, and uniform manifold approximation and projections, and their kidney expression was further confirmed through immunohistochemistry with antibody against vWf (Abcam, Cambridge, the United Kingdom; cat#: ab6994), laminin subunit alpha 3 (LAMA3, MyBioSource; cat#: MBS9238069), intercellular adhesion molecule 1 (ICAM1, MA1-80910, ThermoFisher, Waltham, MA), Tetraspanin 18 (TSPAN18, ThermoFisher; cat#: PA5-48957), and growth hormone secretagogue receptor (GHSR, LSBio, Seattle, WA; cat#: LS-A6576).
Techniques: Expressing